PAMPA—critical factors for better predictions of absorption

PAMPA, log POCT, and Caco‐2 are useful tools in drug discovery for the prediction of oral absorption, brain penetration and for the development of structure‐permeability relationships. Each approach has its advantages and limitations. Selection criteria for methods are based on many different factors: predictability, throughput, cost and personal preferences (people factor). The PAMPA concerns raised by Galinis‐Luciani et al. (Galinis‐Luciani et al., 2007, J Pharm Sci, this issue) are answered by experienced PAMPA practitioners, inventors and developers from diverse research organizations.