Recommended Reading: Absorption using the  PAMPA Approach (in Optimization in Drug Discovery: In Vitro Methods)

Thoroughly  revised and updated, Optimization in Drug Discovery:  In Vitro Methods, Second Edition presents a wide  spectrum of in vitro assays including formulation, plasma binding, absorption  and permeability, cytochrome P450 (CYP) and UDP-glucuronosyltransferases  (UGT) metabolism, CYP inhibition and induction, drug transporters, drug-drug  interactions via assessment of reactive metabolites, genotoxicity, and  chemical and photo-mutagenicity assays. Written for the Methods in Pharmacology and Toxicology series,  chapters include introductions to their respective topics, lists of the  necessary materials and reagents, step-by-step, readily reproducible  protocols, and tips on troubleshooting and avoiding known pitfalls. Expert  authors have developed and utilized these in vitro assays to achieve  “drug-like” characteristics in addition to efficacy properties and good  safety profiles of drug candidates.