AN02 PKa measurements in 15 minutes - the SiriusT3 Fast UV pKa method

Understanding the ionisation properties of a molecule is an essential requirement for drug development. SiriusT3 technology provides the capability to perform pKa measurements in 15 minutes using only 5 µL of a 10 mM stock solution. This method requires that the molecule exhibits discernable UV absorption changes on ionisation and possesses an intrinsic aqueous solubility greater than 30 µM. Compounds having a lower aqueous solubility can be accommodated using a cosolvent assay. The SiriusT3 Fast UV pKa method has been validated against results reported in the literature and produces results that are of comparable quality to those obtained by traditional methods. Measurement repeatability is typically within 0.01 pKa units. A complete summary of the data collected in this study is available on request.