Cell lysis is the process by which a cell’s membrane is ruptured so that the cell’s contents are released. It can occur naturally (such as when certain viruses cause infections that lead to cell rupture and death) or it can be induced artificially. In addition to scientists who wish to study and experiment with intracellular contents, and thus, must be able to control the cell lysis process, there are multiple industries (including, but limited to, the pharmaceutical, biotech, food and chemical industries) that need to lyse cells in order to make products. To ensure their products’ safety, quality and efficacy, these industries must intentionally disrupt cell membranes according to their own standards instead of waiting on any natural lysis sequences that mayor may not occur. Indeed, one of the most critical components of any manufacturing process is ensuring safety, removing all potentially harmful substances so that the wellness of consumers is always protected. To this end, many industries use artificial cell lysis to remove pathogens, of which some of the most frequently occurring are bacterial cells. The question then becomes: what’s the best way to lyse bacterial cells, essentially killing them and rendering them incapable of causing illness and disease? Here’s the short answer:
Bacterial Cell Lysis is Best Facilitated by Pion's BEE Brand High-Pressure Homogenizers
While there are several ways to artificially lyse cells (sonication, chemical lysis and traditional homogenization, to name a few examples), there is only one method that is capable of lysing all cell types (including bacterial ones) and that is high-pressure homogenization. Specifically, our high-pressure homogenizers incorporate a patented Emulsifying Cell (EC) technology that combines shear, cavitation and impact forces so that the high-pressure homogenizing process always renders optimal results. Whether used in research, pilot or industrial settings, Pion's BEE brand high-pressure homogenizers ensure the best processes for bacterial cell lysis because they:
- Provide accessibility of intracellular proteins for extraction and solubilization.
- Disrupt all cell types since users can alter pressure, flow and processing time, as well as the amount of cavitation, shear and impact forces, to get the best results for the type of cell they are disrupting.
- Offer flexibility, allowing easy adjustment for different cell disruption strategies.
- Deliver high yields in less time, which lowers Total Cost of Operation (TCO).
- Possess scalable technology, which enables users to elevate from laboratory scale to pilot scale, while achieving reproducible results.
- Support various sample sizes and permit users to adjust the widest variety of mixing forces.
- Require minimal training and are easy to use, reconfigure, clean and maintain.
Want to Learn More?
To learn more about high-pressure homogenization and why it is the best method for bacterial cell lysis, please contact our team. We believe our equipment offers superior quality and provides the best value for researchers and manufacturers alike. Our proprietary processes guarantee that our customers produce high cell rupture yields in the shortest amount of time across the widest variety of cell types.