Homogenization is a general term that encompasses multiple methods, which include mechanical, freeze-thaw, ultrasonic, and detergent-based lysis. Of these, mechanical homogenization is well-known for its efficiency and ability to alter a sample’s physical structure while maintaining its chemical composition. Rotor-stator and blade-type are classified under the mechanical homogenizer umbrella largely due to their physical blending of ingredients to create a homogenous solution. Additionally, each can be used as a pretreatment of animal tissue and plant tissue prior to high-pressure homogenization. Below we review each of the two methods for their benefits and drawbacks, so that you can make an informed decision about which type is best for your laboratory purposes.
Originally developed to produce emulsions and dispersions, rotor-stator homogenizers are incredibly effective machines that, when used properly, confer high product yield and small particle size. When a sample is processed through the machine, a quickly rotating rotor draws the sample up; it is then pushed in a centrifuge-like fashion through a stator that contains many small slots. The combination of high rpm from the rotor and exit through tiny slots allows the sample to be mechanically sheared into very small particles. To enhance process efficiency, multiple variables can be optimized; these include sample size, rotor tip speed, processing time, and sample viscosity. (1)
In contrast to rotor-stator homogenizers, blade homogenizers are less frequently selected for use because of their comparatively decreased efficiency. Also referred to as blenders, blade homogenizers contain cutting blades that quickly rotate to shear the sample. Although they cannot reduce particle size to the capacity of rotor-stator homogenizers, blade homogenizers are useful in their ability to process a wide range of sample sizes.
Pion: Trustworthy High-Pressure Homogenizers
The product you select for your laboratory will ultimately depend on your downstream applications and the cell types you are using. Either way, you will be well-served to select a homogenizer that is flexible to meet the various needs of a laboratory. There are plenty of companies on the market to select your equipment from; however, the lysate can be of higher quality and more even consistency when run through top-shelf equipment, most frequently in the form of a homogenizer.
Pion's BEE brand technologies is trusted by researchers around the world for both our laboratory homogenizers and our associated customer support. Cell lysis is just one of a variety of applications for Pion's homogenizers; nano/micro emulsions, lipids, suspensions, and dispersions are also easily achievable. Additionally, the homogenizer processes can be controlled to suit your product, which will allow you to customize to your cell type. And finally, the equipment is easy to use, produces higher yield in less time, and achieves results that are reproducible and scalable.
Learn about how Pion's BEE brand products can maximize your homogenization processes by visiting our high pressure homogenization products.