Sonication Cell Lysis: How Does it Compare to Homogenization

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Tal Shechter
Mar 9, 2017
min read
Sonication Cell Lysis: How Does it Compare to Homogenization

Homogenization, also known as micronization or particle size reduction, is an important process step that is utilized in a variety of industries, including pharmaceutical, biotech, cosmetic, and food. With benefits like improved taste, texture, and appearance, its no wonder that more and more industries are looking to purchase a homogenizer for their manufacturing needs!

However, before purchasing your next homogenizer, it is important not only to know what your desired end use is, but the capabilities of the homogenizers in today’s marketplace. One question that is frequently asked regards the capabilities of homogenizers versus a sonicator. If you are interested in learning more about sonication cell lysis and how it compares to homogenization, you are in the right place! Continue reading to learn more about sonication cell lysis, how it compares to homogenization, and what BEE International has to offer in the way of high quality, high pressure homogenization equipment.

Sonication is defined as the act of applying sound energy to agitate particles in a sample. It is used for various purposes, including cell lysis, and it is a very powerful technology with both chemical and physical effects. Sonication serves multiple purposes, and is often used for the production of nanoparticles, such as nanoemulsions, as well as nanocrystals, liposomes, wax emulsions, and extractions of compounds such as plant oils and antioxidants.

Sonication works to lyse cells very quickly, with most methods taking between 15 seconds and 2 minutes. The intensity of sonication is quite easy to adjust, allowing for gentle or abrupt disruption of cell membranes. The temperature and length of sonication can also be adjusted.

Homogenization, on the other hand, involves the forcing of cells or tissue suspensions through a narrow space, which shears the cell membranes. Due to the high pressures associated with homogenization, fewer passes are needed to completely disrupt the cells, making homogenization a quicker (and oftentimes easier) choice for the lysing of cells.

Pion's BEE brand offers a variety of homogenization equipment to suit virtually any need. Our homogenizers are suitable for laboratory and research & development use, as well as pilot plant and industrial use. Our homogenizers also offer the following features and benefits:

  • Easy to clean with CIP (Clean in Place) technology
  • Variable operating pressure from 5,000-45,000 psi
  • Modular design for lower cost replacement parts
  • PLC control and monitoring for simple push-button operation

Please contact us today to learn more about the homogenization equipment that we have to offer. We look forward to working with you to optimize the homogenization process for your industry.

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