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Cell Homogenization: Types of Cells & The Best Way to Lyse Them

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Deb Shechter
|
Oct 28, 2016
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1
min read
Cell Homogenization: Types of Cells & The Best Way to Lyse ThemCell Homogenization: Types of Cells & The Best Way to Lyse ThemCell Homogenization: Types of Cells & The Best Way to Lyse Them

Cell lysis (or cell homogenization) is the rupture of the cell membrane resulting in the release of cell contents, and the subsequent death of the cell. Cell lysis takes place in biotech, pharmaceutical, food, cosmetic, and chemical laboratories every day. Research scientists and laboratory technicians disrupt the cell’s structure in order to extract organelles, proteins, DNA, or mRNA. However, many common cell lysis methods are incomplete, leading to wasted time, excess costs, high risks, and they can even undermine the entire research effort.

A wide variety of cells require rupture such as E. coli, yeast, mammalian tissue, bacteria, algae, fungi and insect cells. The primary difference between plant and animal cells is that plant cells have a cell wall and a cell membrane, while animal cells only have a cell membrane.

Adaptable Cell Homogenization with DeBEE High Pressure Homogenizers

Different types of cells and biological materials require different cell disruption methods. Some only require gentle processing, while others need harsher methods. Sonicators, for example, are unsuitable for lysing certain plant cells, as cell walls are very resistant to sonication. Organic solvents like alcohols, ether or chloroform can disrupt cells by permeating the cell walls and membranes. These solvents are often used, in combination with shearing forces, to lyse plant cells.

Pion's BEE brand offers a flexible approach to cell homogenization, as the process is adjustable to handle a wide variety of cells. Our modular technology allows you the ability to control the pressure in order to rupture different cell types including more challenging cells such as yeast or fungi. Cells are ruptured without damaging the valuable intracellular materials, and without the use of solvents and chemicals.

DeBEE High Pressure Homogenizers use forces like shear, cavitation and impact to disrupt cells. Different cells can be ruptured using the most efficient process for the highest recovery rates.

Advantages of Cell Homogenization with DeBEE High Pressure Homogenizers:

  • Accessibility of intracellular proteins
  • Built-in process flexibility
  • Ease-of-use and low learning curve
  • High yields in less time
  • Reproducible and scalable results
  • Various sample size processing
  • Ability to disrupt all cell types
  • Better results in fewer passes

For a quote on any one of our homogenizer models, contact us today and let us show you the advantage of Pion's BEE brand High Pressure Homogenizers for your application.

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