Cell Disruption Methods: Which One Should You Use?

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Deb Shechter
Mar 8, 2017
min read
Cell Disruption Methods: Which One Should You Use?

Cell disruption is a critical part of the overall homogenization process, and is used by a number of industries, including pharmaceutical, biotech, cosmetic, and food. But where does cell homogenization fit into the overall process of homogenization? What are the different methods of cell disruption? And most importantly, which method should you use? Continue reading for the answers to all of those questions, as well as more information on Pion and our suite of high quality homogenization equipment.

Homogenization is also known as micronization, or more simply, particle size reduction. However, before the desired particles within the cell can be released and studied, another step must first take place: cell disruption. Cell disruption is defined as the act of breaking particles apart.

There are numerous forces at play, all of which can be leveraged to disrupt and lyse cells. These forces include impact, shear, turbulence, and cavitation. The type of cell that you wish to disrupt helps drive the decision on which force should be used.

For example, a particle with a harder cell wall, such as yeast, sees the best results with the use of the force of impact. On the other hand, a more delicate particle, such as E. Coli, would benefit from a gentler force such as shear. Other cell types see the best results when utilizing turbulence or cavitation.

When selecting the best homogenization equipment for the task at hand, it is important to have a general understanding of the different types of forces, as well as the forces that should be used for your particular application. This is where Pion comes in. Our technology allows for the use of multiple forces that can be tweaked and adjusted to yield the highest quality results.

Our equipment offers numerous benefits to the end consumer. Our homogenizers are easy to use, produce a higher yield in less time than our competitors, and allows for the production of repeatable, reliable, and scalable results.

Pion's BEE brand offers a number of high quality homogenizers for virtually every industry and setting – from a small laboratory or research and development setting all the way up to pilot plants and industrial manufacturing environments. Our homogenizers offer these additional benefits and features as well:

  • Ability to control pressure to rupture a variety of cell types
  • Ability to rupture cells without damaging the intracellular materials
  • Better results in fewer passes

To learn more about the homogenization equipment that Pion has to offer, please contact us today. We would be more than happy to assist you with your next manufacturing project.

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