Microbial cells are used by researchers around the world, every single day. Their simple forms, ability to grow quickly in vitro, and ease in experimentation makes them ideal candidates for the job. Disruption of these cells, while not comparatively complicated, can feel so when using a new process and machinery. To help even the inexperienced user navigate the world of microbial homogenization, below are some key tips to bear in mind. Happy homogenization!
- There is no universally optimized design. As easy as it would be to find a single experimental design and homogenizer system that work for every application, that is not necessarily a reality. When shopping around for homogenizer systems it is important to seek out one that is both scalable and customizable; this will allow the user much more flexibility in its use.
- Cell analysis requires specific microscope access. Analysis of the homogenate will require a microscope, specifically one with phase-contrast options and a spectrophotometer. A scientist can thus use a dye-binding assay with the spectrophotometer, which measures protein release concentration, to determine the level of disruption.
- Analysis of the homogenate should occur as soon as possible. Of course, contamination is an increasing risk as a bacterial sample sits out longer. But just as importantly, after disruption, viable cells that linger in the broth may reproduce using substrates recycled from disrupted cells.
- Cleanability of the homogenizer should be carefully considered. If not cleaned properly after use, a homogenizer can provide the grounds for contamination of future samples. As such, a machine’s cleanability should be considered; specifically, it should be easy to perform regular maintenance and users should be diligent about following the manufacturer’s recommended maintenance schedule. Additionally, users should determine if they are comfortable with a product that requires disassembly.
Pion: BEE Brand Homogenizers That Effectively Disrupt Cells
High pressure homogenization (HPH) is the most commonly used method for microbial cell disruption due largely to its effectiveness at the medium and large scale. Not only can its techniques be scaled to any sample size, but its settings are also customizable to most cell types. On determining which homogenizer will be the best fit, the search can begin with Pion. We are globally recognized among laboratory managers and researchers for their high-quality products and excellent customer support.
Cell lysis is just one of a variety of applications for our homogenizers; nano/micro emulsions, lipids, suspensions, and dispersions are also easily achievable. Additionally, the homogenizer processes can be controlled to suit any given product, which will allow for customization to the cell type. And finally, the equipment is easy to use, produces higher yield in less time, and achieves results that are reproducible and scalable.