BioFLUX
BioFlux™ system can be combined with the in situ Fiber Optic UV System to study absorption potential of drugs final dosages.
PAMPA
Pion’s PAMPA is an in vitro, high throughput screening assay highly correlated to biological membrane permeability.
LOREM IPSUM
Screening excipients, solvents, and APIs to rank order formulations and drug candidates on the basis of permeability.
LOREM IPSUM
Each PAMPA well uses artificial lipid membranes that mimic drug uptake in the gastrointestinal tract (GIT) or the blood-brain barrier(BBB) to provide robust, reliable, bio relevant results.
BioFlux™ system can be combined with the in situ Fiber Optic UV System to study absorption potential of drugs final dosages.
Used for conducting simultaneous dissolution-absorption studies.
Assess the complex of interplay between solubility, permeability and dissolution rate in-vitro.
Evaluate whether certain formulation changes will affect the bio-performance of a certain drug product.
The In-Situ UV Probe-Based Dissolution System
The BioFLUX™ system consists of an absorption (receiver) chamber integrated with a lipophilic membrane, overhead stirrer and fiber optic (FO) UV probe that is inserted in to a modified cover of a miniaturized 500 mL vessel of USP II dissolution apparatus.
The phospholipid-covered artificial permeation membrane separates the dissolution (donor) compartment from the receiver compartment, containing Pion Acceptor Sink Buffer at pH 7.4. The dissolution compartment typically holds 200 – 250 mL of compendial or biorelevant dissolution buffer. The modified vessel cover allows fiber-optic UV probes to be positioned in both the donor and receiver compartments to monitor real-time concentration kinetic in both chambers. Concentration monitoring is enabled by connecting the fiber optic UV probes to the Rainbow Dynamic Dissolution Monitor® instrument.
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THE PION PAMPA ADVANTAGE
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In Vivo Correlation
Gut-Box controls stirring speed to match the hydrodynamics of the intestinal tract or the blood brain barrier. The adjustable stirring allows you to mimic the ABL (aqueous boundary layer), allowing for higher accuracy and reducing assay time.[1].
High Throughput
The STIRWELL plates in Pions’ PAMPA sandwich kits contain a pre-loaded stir bar in each well, providing dynamic stirring for each assay, allowing for high-throughput screening of multiple compounds simultaneously.
Easy Data Interpretation
PAMPA Explorer software for easy data
interpretation. SW includes a plate-reader
data wizard, results visualization, and reporting. Data output is the complete mass balance including membrane retention.
Reproducible Results
While orbital shakers can lead to edge
effects in the assay plates, GutBox provides consistent agitation across the whole plate, allowing for more reproducible data.
Complete Solution
The complete PAMPA system includes our 96well STIRWELL plates, the Gut Box™ stirring system and PAMPA Explorer™ software.
Pion’s PAMPA is an in vitro, high throughput screening assay highly correlated to biological membrane permeability
Screening excipients, solvents, and APIs to rank order formulations and drug candidates on the basis of permeability.
Each PAMPA well uses artificial lipid membranes that mimic drug uptake in the gastrointestinal tract (GIT) or the blood-brain barrier(BBB) to provide robust, reliable, bio relevant results.
Improve Process and Decision Making with Better In Vitro Insights
The BioFLUX™ in conjunction with Rainbow R6
- Provided valuable information about permeability-solubility interplay of poorly soluble drugs during formulation development;
- Allows to perform experiments at biorelevant volumes (200-250mL) for late stage formulation development or bioequivalence studies;
- Designed to work with USP II apparatuses.
BioFLUX
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