PAMPA Explorer Frontier™

PAMPA Explorer Frontier is the perfect solution for researchers who want to measure permeability in their lab.

 

This comprehensive system provides all that is needed to confidently and easily run dozens of permeability assays per day.

 

We can also supply you with a compatible UV reader.

 

                           

 

PAMPA Models

 

Gastrointestinal Permeability  The gastrointestinal tract (GIT) has a pH range from pH 1 – 8.  Since the pH of the blood is pH 7.4; there exists a pH gradient between the GIT and the plasma that can affect the transport of ionizable molecules. In an effort to simulate this pH gradient, we have chosen pH 7.4 for the acceptor compartment, and a pH range of 5.0, 6.2, and 7.4 in the donor compartment. To model transport conditions in the blood, the acceptor contains a scavenger binding molecule. Pion’s GIT-0 phospholipid is used for the assay.      

Blood-Brain Barrier Permeability  CNS Screening of candidate drug molecules is done using a PAMPA assay to model blood-brain barrier (BBB) permeability. Rodent in vivo and in situ studies of the kinetics of drug uptake across the BBB are valuable tools for assessing factors important to steady-state brain penetration. These are relatively expensive and time consuming assays to do, and are done only sparingly. Animal studies can be augmented by PAMPA, which for BBB uses a mixture of phospholipids infused into lipophilic microfilters, with net negative lipid charge, a system mimicking many of the properties of brain lipid membranes. The data is used to predict brain uptake kinetics, as indicated by PS values determined by in situ perfusion methods.

 

 

 

Skin Penetration  Researchers and product developers can now measure drug permeability with a proprietary test system that is highly predictive of the human skin barrier.  Unlike conventional skin test methods, Pion’s high throughput method is faster, less expensive and may be conducted manually or on robotic platforms. 

 

The Skin PAMPA Test System excels as a complimentary screen prior to Franz Cell testing due to the lower cost, enhanced usability, higher throughput and reduced sample requirements.  Pre-coated with a proprietary formulation, the 96 well plates are disposable, which completely eliminates cross contamination and the additional time and labor required for washing glass cells.  Permeability measurements with the artificial skin-mimetic membrane exhibit a high correlation as tested against human skin in Franz cells.

 

 

Features

 

  • Save valuable research time and expensive in vivo testing
  • Determine permeability with sub-micromolar detection limit – saving precious API
  • Easy to use – quickly start measuring permeability
  • Instantly analyze data – using a user-friendly interface software with advanced data processing
  • Generate unique and creative models using Pion ’s open platform
  • Don’t be confined to traditional methods – STIRWELL™ Plates provide you with the flexibility to customize your assay design
  • Be confident – Obtain consistent reproducible results with validated artificial models

 

 

Which PAMPA System is Right for You?

 

The PAMPA Explorer Frontier combines the science of µSOL and its Explorer Command Software with the versatility of the the Gut-Box™. We also have the PAMPA Evolution™, which is the highest integration level robotic platform.

 

Additional Resources