PAMPA Evolution™

Pion's PAMPA Evolution™ is an in vitro instrument for measuring drug permeability using the PAMPA method.

 

Based on a 96-well microtiter plate format, this system will allow you to screen compounds and effectively predict their permeability. Leveraging our expertise in absorption prediction through the GITBBB, and Skin barriers will get your product to market faster, without compromising the science.

 

Our PAMPA Evolution software will integrate with any automated liquid handling robotic platform—Tecan, Beckman, Hamilton, etc—and with our stirring device, the Gut-Box. It will execute hundreds of permeability assays per day, allowing you to get reliable, fast and reproducible results for your compounds.

 

Liquid handling scripts automated with PAMPA Evolution are available on the Tecan Freedom EVO® for use with Infinite™ M200 or M1000 plate readers, and the Beckman Biomek® FX robotic platforms with a Molecular Devices SpectraMax Reader.    

 

 

PAMPA Models

 

Gastrointestinal Permeability  The gastrointestinal tract (GIT) has a pH range from pH 1 – 8.  Since the pH of the blood is pH 7.4; there exists a pH gradient between the GIT and the plasma that can affect the transport of ionizable molecules. In an effort to simulate this pH gradient, we have chosen pH 7.4 for the acceptor compartment, and a pH range of 5.0, 6.2, and 7.4 in the donor compartment. To model transport conditions in the blood, the acceptor contains a scavenger binding molecule. Pion’s GIT-0 phospholipid is used for the assay.      

Blood-Brain Barrier Permeability  CNS Screening of candidate drug molecules is done using a PAMPA assay to model blood-brain barrier (BBB) permeability. Rodent in vivo and in situ studies of the kinetics of drug uptake across the BBB are valuable tools for assessing factors important to steady-state brain penetration. These are relatively expensive and time consuming assays to do, and are done only sparingly. Animal studies can be augmented by PAMPA, which for BBB uses a mixture of phospholipids infused into lipophilic microfilters, with net negative lipid charge, a system mimicking many of the properties of brain lipid membranes. The data is used to predict brain uptake kinetics, as indicated by PS values determined by in situ perfusion methods.

 

 

 

Skin Penetration  Researchers and product developers can now measure drug permeability with a proprietary test system that is highly predictive of the human skin barrier.  Unlike conventional skin test methods, Pion’s high throughput method is faster, less expensive and may be conducted manually or on robotic platforms. 

 

The Skin PAMPA Test System excels as a complimentary screen prior to Franz Cell testing due to the lower cost, enhanced usability, higher throughput and reduced sample requirements.  Pre-coated with a proprietary formulation, the 96 well plates are disposable, which completely eliminates cross contamination and the additional time and labor required for washing glass cells.  Permeability measurements with the artificial skin-mimetic membrane exhibit a high correlation as tested against human skin in Franz cells.

 

 

Features 

  • Direct permeability units (10-6 cm/s)
  • Five orders of magnitude resolution
  • Membrane retention is assessed
  • Unstirred water layer reduction with Gut-Box
  • Sink conditions not assumed in calculations
  • Constants corrected for ionization when pKa is known
  • High-throughput (parallel UV detection)
  • Shape spectral analysis for data quality evaluation
  • LC/MS interface for external detection import
  • Open access

 

Which PAMPA System is Right for You?

 

The PAMPA Evolution™ is the highest integration level robotic platform. We also have the PAMPA Explorer Frontier, which combines the science of µSOL and its Explorer Command Software with the versatility of the the Gut-Box™

 

Additional Resources