Permeability

Using Pion CRO Services is the fastest, and most cost-effective way to get permeability predictions for your compounds. Leveraging our unparalleled expertise in absorption measurements will get you reliable and reproducible results

 

  

                                                                    What is PAMPA?

 

                                             Parallel Artificial Membrane Permeability Assay       

 

                         

 

Reduce in vivo testing                             Less than $2 per test

                   API Sparing on µL scale                           Reproducible & Flexible 

 

 

 

Can your in vitro assay account for:

 

  • Biorelevant Lipid Composition?
  • pH Gradient (pH 3-10)?
  • Sink Conditions (Binding to serum proteins)?
  • Lipid Charge?
  • Unstirred water layer?

 

The Pion PAMPA assay is used as an in vitro model of passive, transcellular permeability. An artificial membrane immobilized on a filter is placed between a donor and acceptor compartments.  At the start of the test, a drug is introduced in the donor compartment. Following the permeation period, the concentration of drug in the donor and acceptor compartments is measured.


Pion has over 25 publications on PAMPA in peer-reviewed journals in collaboration with academics and pharmaceutical scientists from the industry including Dr. Manfred Kansy (Hoffman-La Roche, CH), founder of the PAMPA method.
 

Visit our LIBRARY for a full list of PAMPA Publications

 

                                        

                                                            

 

                                                                      Pion PAMPA Models

Gastrointestinal Permeability  The gastrointestinal tract (GIT) has a pH range from pH 1 – 8.  Since the pH of the blood is pH 7.4; there exists a pH gradient between the GIT and the plasma that can affect the transport of ionizable molecules. In an effort to simulate this pH gradient, we have chosen pH 7.4 for the acceptor compartment, and a pH range of 5.0, 6.2, and 7.4 in the donor compartment. To model transport conditions in the blood, the acceptor contains a scavenger binding molecule. Pion’s GIT-0 phospholipid is used for the assay.

 

                                               

Blood-Brain Barrier Permeability  CNS Screening of candidate drug molecules is done using a PAMPA assay to model blood-brain barrier (BBB) permeability. Rodent in vivo and in situ studies of the kinetics of drug uptake across the BBB are valuable tools for assessing factors important to steady-state brain penetration. These are relatively expensive and time consuming assays to do, and are done only sparingly. Animal studies can be augmented by PAMPA, which for BBB uses a mixture of phospholipids infused into lipophilic microfilters, with net negative lipid charge, a system mimicking many of the properties of brain lipid membranes. The data is used to predict brain uptake kinetics, as indicated by PS values determined by in situ perfusion methods.

 

                                                

 

 

Skin Penetration  Researchers and product developers can now measure drug permeability with a proprietary test system that is highly predictive of the human skin barrier.  Unlike conventional skin test methods, Pion’s high throughput method is faster, less expensive and may be conducted manually or on robotic platforms. 

 

The Skin PAMPA Test System excels as a complimentary screen prior to Franz Cell testing due to the lower cost, enhanced usability, higher throughput and reduced sample requirements.  Pre-coated with a proprietary formulation, the 96 well plates are disposable, which completely eliminates cross contamination and the additional time and labor required for washing glass cells.  Permeability measurements with the artificial skin-mimetic membrane exhibit a high correlation as tested against human skin in Franz cells.