The gastrointestinal tract (GT) has a pH range from pH 1 – 8. The pH of the blood is constant at pH 7.4; therefore it is possible for a pH gradient to exist between the GT and the plasma that can affect the transport of ionizable molecules. In an effort to create such gradient pH conditions, we have chosen pH 7.4 for the acceptor compartment and pHs 5.0, 6.2, and 7.4 in the donor compartment. To model transport conditions in the blood, the acceptor contains a scavenger chemical. pION’s GIT-0 phospholipid is used for the assay.

In the PAMPA method, an artificial membrane immobilized on a filter is placed between a donor and acceptor compartment. The drug is introduced in the donor compartment. Following the permeation period, the donor and acceptor compartments are quantified using UV spectroscopy. Since this method does not use potentiometry, any compound with UV absorption may be submitted for permeability analysis. To request the analysis, please use the Sample Submittal Sheet provided below.

• Fill out the sample submittal sheet (can be downloaded below)
• Obtain a purchase order number and include the number on the sheet
• Fill out the Sample Input Template (can be downloaded below) and email to analytical.service@pion-inc.com
• Send 125 µL of your samples as a 10-100 mM DMSO solution in a frozen microtitre plate. Alternatively, 5-7 mg of solid sample may be submitted.*

* Additional sample preparation charge applies.

GIT Permeability Sample Submittal Sheet (PDF)

BBB Permeability Sample Submittal Sheet (PDF)

Sample Input Template (XLS)

© 2001-2007 pION INC. All rights reserved. Copyright Statement