Permeability Workshops: Foundations and Practical Aspects of PAMPA, Oct 6-8, 2010

Who would benefit: 

Everyone using or considering using artificial membrane permeability assays:

PAMPA Evolution/PAMPA Explorer users, researchers involved in permeability and absorption studies, supervisors wanting to learn more about the bio-physics of permeability and its role in the overall set of absorption properties, medicinal chemists interpreting permeability results and modifying molecules to improve their drugability, pre-formulators studying the effects of delivery vehicles on absorption of compounds, etc.

What will be covered:         

Overview of the latest developments in PAMPA technology: Double-Sink™, stirring, cosolvents, correcting for aqueous boundary layer effect, the BBB model, PAMPA Excipient screening, etc.
PAMPA and human absorption. BBB PAMPA and rodent in situ brain perfusion experiments.

Proper planning of PAMPA experiments. How to use prediction software to select optimal conditions (pH range, stirring, incubation time). Case studies: When will thoughtful planning help in obtaining the best quality PAMPA data. Interactive practice of the art.

Industry perspective on PAMPA: learn from your colleagues how they are using PAMPA data in their companies.

Hands on practice on setting up PAMPA experiments for those who have never done it before. Processing permeability data with PAMPA Explorer/PAMPA Evolution Command Software. Extracting the most and best information from your data. Interactive analysis of PAMPA data sets. How to recognize bad data and how to recover decent results even from less than perfect data sets. When to use different permeability models: iso-pH, gradient-pH, sink conditions. Exporting/importing spectral and permeability data.

Recognizing experimental limitations imposed by the unstirred water layer effect and how to correct for it.Detection systems. How to interpret UV data. When LC/MS is necessary. How to bring external detection data into PAMPA processing software.

 Who Conducts the Workshop:

Konstantin Tsinman, PhD: Manager, Instrumentation & Software Development

Dr. Tsinman is a Physicist and Analytical Chemist, with over 10 years experience in developing analytical instruments used in drug development. He currently is the Project Leader for a 3 year NIH SBIR grant to develop a high-throughput instrument to measure and model blood-brain barrier permeability. Previously at pION, he was the Principal Designer of the first commercial high-throughput PAMPA (parallel artificial membrane permeability assay) instrument and high-throughput solubility analyzer. Dr. Tsinman has authored more than 30 scientific publications and is co-author of 2 patents.

Oksana Tsinman: Research Chemist / R&D

Many of you know Oksana already. She has everyday, hands on experience with PAMPA and Caco-2 assays. You will get the kind of practical advice, hints and tips that just do not come with a manual.

 Alex Avdeef, PhD: CEO / CSO

Dr. Avdeef will also be on hand. Having over 30 years of experience in chemical instrumentation and software development, he is an internationally known authority on solution chemistry with outstanding academic credentials and an extensive list of publications. His accomplishments in the development of instrumentation include several well-known instruments currently manufactured by leading companies in the instrument market. Dr. Avdeef is an author of a well known book Absorption and Drug Development, Wiley-Interscience: New York, 2003, and has more than 100 publications in peer-reviewed journals. 

What will you bring back:    

A software/reagents/consumables kit to perform Double-Sink™ PAMPA in your lab; practical tips on how to set up a good PAMPA assay; a much deeper understanding of the passive permeability phenomenon and its role in absorption; the ability to properly interpret PAMPA data; and the advantage of personal interaction with other scientists practicing PAMPA. 

A registration form is available for Oct 6-8.

Registration $1800

Where: 

pION INC
5 Constitution Way
Woburn, MA 01801